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Biotium
"Light-on" LysoView™ 555

SKU

70060

Size

50uL

$135

Description & Target

Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is UV-activated and reversible.

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"Light-on" LysoView™ 555, Trial Size

SKU

70060-T

Size

10uL

$43

Description & Target

Light-On LysoView™ 555 is a red fluorogenic lysosome dye with pH-dependent fluorescence. Light-On LysoView™ dye is unique among commercially available lysosomotropic dyes in that its fluorescence in cells is UV-activated and reversible.

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1 kb DNA Ladder in TE buffer

SKU

31039

Size

300uL

$89

Description & Target

The 1 kb DNA Ladder is suitable for sizing linear double-stranded DNA fragments from 250 bp to 10 kb. The ladder is composed of chromatography-purified DNA fragments dissolved in TE buffer (10 mM Tris pH 7.5, 1 mM EDTA) at 100 ng/uL. The 1 kb, 3 kb, and 6 kb bands contain more DNA to provide internal orientation. The ladder is supplied with 1.5 mL of 6X  Loading Buffer, which contains density agents and two blue electrophoresis tracking dyes that run at approximately 1.5 kb and 200 bp in a 1% agarose gel.



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1,6-Dipheny-1,3,5-hexatriene

SKU

60021

Size

100MG

$50

Description & Target

1,6-Diphenyl-1,3,5-hexatriene (DPH, excitation/emission=350 nm/420 nm) is almost non-fluorescent in water, but it exhibits a strong increase in fluorescence after intercalation into membranes and can be used as a probe for viscosity, polarity and lipid order. DPH has been used for the high-content imaging of neutral lipid droplets<sup>1</sup> as well as a reporter of inner spore membrane fluidity in bacterial spores<sup>2</sup>.
<ul>
<li>Yellow solid soluble in DMF and DMSO</li>
<li>Store at -20 °C and protect from light</li>
<li>C<sub>18</sub>H<sub>16</sub></li>
<li>MWt: 232.32</li>
<li>[1720-32-7]</li>
</ul>

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10-Acetyl-3,7-dihydroxyphenoxazine

SKU

10061

Size

5MG

$149

Description & Target

10-Acetyl-3,7-dihydroxyphenoxazine (N-Acetyl-3,7-dihydroxyphenoxazine or ADHP), which has been marketed under the name Amplex&reg; Red, is regarded as the best fluorogenic substrate for peroxidase because it is highly specific and stable. The substrate itself is nearly colorless and nonfluorescent until it is oxidized by H<sub>2</sub>O<sub>2</sub>(reacting in a 1:1 stoichiometry) in the presence of horseradish peroxidase (HRP) to become the highly red fluorescent resorufin. H<sub>2</sub>O<sub>2</sub> is produced by many enzymatic reactions, and thereforeADHP can be used to detect the activity of many different enzymes.<ul> <li>Detect HRP in immunoassays</li> <li>Detect trace amounts of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>)</li> <li>Detect using fluorometer or microplate reader</li> <li>&lambda;<sub>Ex</sub>/&lambda;<sub>Em</sub>(pH 9)= 571/585 nm (end product)</li> <li>&epsilon;= 54,000 (end product)</li> <li>White to off-white solid soluble in DMSO or DMF</li> <li>Store desiccated at -20&deg;C and protect from light</li> <li>C<sub>14</sub>H<sub>11</sub>CNO<sub>4</sub></li> <li>MW: 257.24</li> <li>[119171-73-2]</li></ul>Amplex Red is a registered trademark of Thermo Fisher Scientific.

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10X Fish Gelatin Blocking Agent

SKU

22010

Size

100ML

$61

Description & Target

10X Fish Gelatin Blocking Agent is a solution of cold water fish skin gelatin that can be added to blocking buffers to minimize non-specific a4d73786-81ea-96bb-77d4-9ff261b5ff15 binding in immunodetection procedures such as Western Blotting and immunostaining. Unlike BSA or milk, fish gelatin does not contain IgG or serum proteins that could cross-react with mammalian antibodies. Fish gelatin can be used to block non-specific binding sites on positively charged nylon or PVDF membranes or used for blocking and dilution of antibodies during immunostaining procedures.

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1X Passive Lysis Buffer 2.0

SKU

99821-100ML

Size

100ML

$33

Description & Target

1X Passive Lysis Buffer 2.0 for 9689c5aa-6556-4b3c-b5d1-1e693715ba07’s Firefly & Renilla Luciferase Single Tube Assay Kits (catalog no. 30081) is sold separately for use with the kits when more lysis buffer (e.g. >100 uL/well) is required. It also can be used with recombinant firefly or Renilla luciferase enzymes.

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2',7'-DICHLORODIHYDROFluorescein DIACETA

SKU

10058

Size

100MG

$72

Description & Target

H<sub>2</sub>DCFDA (2',7'-Dichlorodihydrofluorescein diacetate) is a useful fluorogenic reagent to detect reactive oxygen intermediates in cells. On oxidation, H<sub>2</sub>DCFDA becomes the highly green fluorescent 2',7'-dichlorofluorescein.<ul><li>&lambda;<sub>Ex</sub>/&lambda; <sub>Em </sub>(pH&gt;6) = 504/529 nm (end product)</li><li>White solid soluble in DMSO or DMF</li><li>Store desiccated at -20&deg;C and protect from light</li><li>C<sub>24</sub>H<sub>16</sub>Cl<sub>2</sub>O<sub>7</sub></li><li>MW: 487.29</li><li>[4091-99-0]</li></ul>

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2,3-Diaminonaphthalene

SKU

301

Size

100MG

$52

Description & Target

2,3-Diaminonaphthalene reacts with nitrosonium, which is formed from NO, to form the fluorescent dye 1 <i>H</i>-naphthotriazole. This method can be used to detect 10 nM to 10 uM of nitrite (NO<sub>2</sub>-) and is compatible with 96-well format. <ul> <li>Yellow orange solid soluble in DMSO</li> <li>Store at desiccated at 4&deg;C, protect from light</li> <li>C<sub>10</sub>H<sub>10</sub>N<sub>2</sub></li> <li>MW: 158</li> </ul>

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2-((4-AZIDO-2,3,5,6-TETRAFLUOROBENZOYL)A

SKU

91032

Size

10MG

$94

Description & Target

2-((4-Azido-2,3,5,6-tetrafluorobenzoyl)amino)ethyl methanethiosulfonate is a cross-linker containing a thiol-reactive MTS group and a photoaffinity label 4-azido-2,3,5,6-tetrafluorobenzene (ATFB). ATFB is known to be a superior photoaffinity label that undergoes highly efficient C-H bond insertion on UV photolysis. <ul> <li>White solid soluble in DMF or DMSO</li> <li>Store at -20°C and protect from light</li> <li>C<sub>10</sub>H<sub>8</sub>F<sub>4</sub>N<sub>4</sub>O<sub>3</sub>S<sub>2</sub></li> <li>MW: 372.32</li> </ul>

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2-Sulforhodamine 101 cadaverine, trifluoroacete salt

SKU

90115

Size

5MG

$284

Description & Target

2-Sulforhodamine 101 cadaverine can be coupled to activated carboxy groups or sulfonyl chlorides. <ul> <li>&lamba;<sub>Ex</sub>/&lamba;<sub>Em </sub>(MeOH) = 583/603 nm</li> <li>Dark purple solid soluble in DMSO or DMF</li> <li>Store at 4°C and protect from light</li> <li>C<sub>38</sub>H<sub>43</sub>F<sub>3</sub>N<sub>4</sub>O<sub>8</sub>S<sub>2</sub></li> <li>MW: 804.90</li> </ul>

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3,6,9,12-TETRAOXATETRADECANE-1,14-DIYL D

SKU

91044

Size

10MG

$98

Description & Target

3,6,9,12-Tetraoxatetradecane-1,14-diyl dimethanethiosulfonate is a water soluble MTS cross-linker. Stock solutions made in anhydrous DMF are stable for at least two months if stored at -20°C. However, stock solutions made in water or buffer should be used immediately.

<ul>

<li>Off-white solid soluble in DMF or water</li>

<li>Store desiccated at -20°C</li>

<li>C<sub>12</sub>H<sub>26</sub>O<sub>8</sub>S<sub>4</sub></li>

<li>MW: 426.57</li>

</ul>

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3,6,9-TRIOXAUNDECANE-1,11-DIYL DIMETHANE

SKU

91043

Size

10MG

$94

Description & Target

3,6,9-Trioxaundecane-1,11-diyl dimethanethiosulfonate is a water soluble MTS cross-linker. Stock solutions made in anhydrous DMF are stable for at least two months if stored at -20°C. However, stock solutions made in water or buffer should be used immediately.

<ul>

<li>Off-white solid soluble in DMF or water</li>

<li>Store desiccated at -20°C</li>

<li>C<sub>10</sub>H<sub>22</sub>O<sub>7</sub>S<sub>4</sub></li>

<li>MW: 382.52</li>

</ul>

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3,6-DIOXAOCTANE-1,8-DIYL DIMETHANETHIOSU

SKU

91042

Size

10MG

$98

Description & Target

3,6-dioxaoctane-1,8-diyl dimethanethiosulfonate is a water soluble MTS cross-linker. Stock solutions made in anhydrous DMF are stable for at least two months if stored at -20°C. However, stock solutions made in water or a buffer should be used immediately.

<ul>

<li>Off-white liquid soluble in DMF or water</li>

<li>Store desiccated at -20°C</li>

<li>C<sub>8</sub>H<sub>18</sub>O<sub>6</sub>S<sub>4</sub></li>

<li>MW: 338.49</li>

</ul>

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4-Bromo A-23187, free acid

SKU

59006

Size

1MG

$155

Description & Target

Similar to A-23187, 4-Bromo A-23187 is used for in situ calibrations of fluorescent Ca<sup>2 </sup> indicators to equilibrate intracellular and extracellular calcium concentrations and to allow Mn<sup>2 </sup> to enter cells to quench indicator fluorescence. However, since 4-bromo A-23187 is nonfluorescent, it is preferred over the blue fluorescent A-23187 for calibrations of UV-excited Ca<sup>2 </sup> indicators such as Indo-1 and Fura-2 in order to keep background fluorescence to a minimum.
<ul>
<li>White solid soluble in DMSO or EtOH</li>
<li>Store at 4°C</li>
<li>C<sub>29</sub>H<sub>36</sub>BrN<sub>3</sub>O<sub>6</sub></li>
<li>MW: 602.52</li>
<li>[76455-48-6]</li>
</ul>

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