Antibodies
Diagnostic & Veterinary
Supplier
Biotium
Biotin-16-dUTP, 1 mM In Ph 7.5 Tris-HCl

SKU

40022

Size

Each

$226

Descrition & Target

Biotin-16-dUTP can be enzymatically incorporated into DNA via nick translation, random priming, or 3' end terminal labeling. The terminal deoxynucleotidyl transferase (TdT)-mediated biotin-dUTP nick end-labeling (TUNEL) method has been commonly used for apoptosis studies. The number '16' is the number of atoms in the linker between biotin and dUTP. 9689c5aa-6556-4b3c-b5d1-1e693715ba07 also offers biotin-11-dUTP and biotin-20-dUTP. The length of the linker affects the incorporation efficiency of the biotin-dUTP probe into DNA using DNA polymerases, and it also affects biotin/avidin or biotin/streptavidin. In general, the shorter the linker, the more efficiently the biotin-dUTP is incorporated into DNA by DNA polymerases. On the other hand, a longer linker should facilitate interaction between biotin and avidin or streptavidin.
<ul>
<li>1 mM solution in pH 7.5 Tris-HCl buffer</li>
<li>Store at -20°C</li>
<li>C<sub>32</sub>H<sub>48</sub>N<sub>7</sub>O<sub>18</sub>P<sub>3</sub>Sli<sub>4</sub></li>
<li>MW: 971.5</li>
</ul>
Biotin-16-dUTP is also available as a lyophilized solid.

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