Fluorescent Labeling:Options & Optimization

Fluorescent Labeling: Options & Optimization Even aside from primary factors such as color and affinity, selecting an ideal fluorescent dye in the laboratory often includes attention to current imaging equipment, price, and other practical concerns. For exact comparisons with older publications, Alexa Fluor® conjugates and analogs are a common choice. The expanded iFluor™ series are a popular selection for scientists prioritizing photostability and brightness. For affordability, classic dyes like fluoresceins, cyanines, and others are useful on a large scale, for a moderate loss of performance. Laboratories with time constraints might be best served by antibody and protein labeling kits, designed for easy use with minimal training required. Choose the best option to fit your needs, or contact us for assistance with dye selection. Click To Explore The iFluor™ Catalog

HeLa cells stained with mouse anti-tubulin followed with iFluor™ 594 goat anti-mouse IgG. Actin filaments were stained with Phalloidin-iFluor™ 488 Conjugate and nuclei were stained with DAPI.

Featured Resources Many resources and specialized products for researchers are available, from Application Notes to open-access tools and protocols. See the comparison table at right for label equivalents, or consult the iFluor™ Dye Selection Guide.

IHC Detection & Amplification:

Immunohistochemistry (IHC) Catalog

Recently Added To Catalog

BSA-Compatible ReadiLink™ xtra Rapid Antibody Labeling Kits:

No Purification Column Required

~10 Minutes Hands-On Time

Online Fluorescence Spectrum Viewer

AAT Bioquest's Interactive Spectrum Viewer is a powerful tool for analysis and comparison of the spectra of common compounds used in absorbance and fluorescence formats.

If You Use: Alexa Fluor® 350, AMCA, coumarin

Try The Equivalent: iFluor™ 350

If You Use: HiLyte™ 488, Cy2, FITC

Try The Equivalent: iFluor™ 488

If You Use: Alexa Fluor® 555, HiLyte™ 555, Cy3, TRITC

Try The Equivalent: iFluor™ 555

If You Use: Alexa Fluor® 568, Atto 565, Rhodamine Red

Try The Equivalent:iFluor™ 568

If You Use: Alexa Fluor® 594, Atto 594, Texas Red

Try The Equivalent: iFluor™ 594

If You Use: Alexa Fluor® 647, Atto 647, HiLyte™ 647, Cy5

Try The Equivalent: iFluor™ 647

If You Use: Alexa Fluor® 680, Atto 680 Cy5.5, IRDye® 680

Try The Equivalent: iFluor™ 680

If You Use:

Alexa Fluor® 750, HiLyte™ 750, Cy7

Try The Equivalent:iFluor™ 750

Alexa Fluor® is a registered trademark of ThermoFisher. HiLyte™ is a trademark of AnaSpec.

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